About Us

Shattuck is a clinical-stage biotechnology company pioneering the development of bi-functional fusion proteins as a new class of biologic medicine for the treatment of patients with cancer and autoimmune disease. Compounds derived from Shattuck’s proprietary Agonist Redirected Checkpoint, ARC®, platform simultaneously inhibit checkpoint molecules and activate costimulatory molecules within a single therapeutic. The company’s lead wholly owned program, SL-172154 (SIRPα-Fc-CD40L), which is designed to block the CD47 immune checkpoint and simultaneously agonize the CD40 pathway, is being evaluated in a Phase 1 trial. A second compound, SL-279252 (PD1-Fc-OX40L), is being evaluated in a Phase 1 trial in collaboration with Takeda Pharmaceuticals. Additionally, the company is advancing a proprietary Gamma Delta T Cell Engager, GADLEN™, platform, which is designed to bridge gamma delta T cells to tumor antigens for the treatment of patients with cancer. Shattuck has offices in both Austin, Texas and Durham, North Carolina. For more information, please visit: www.ShattuckLabs.com

 

About the Position

The Cell Line Development (CLD) vector engineering Scientist will lead the development of a panel of expression vectors for generation of high yielding stable CHO cell lines. Responsibilities will include design and evaluation of single and double gene vectors for use in generation of high yielding stable cell lines expressing novel bi-functional recombinant proteins developed using Agonist Redirected Checkpoint (ARC) technology.

 

Summary of Key Responsibilities

  • Lead CHO-DG44 host cell development and banking for use in CLD
  • Suspension adapt parental host cells in serum free scalable culture conditions
  • Develop novel CHO-DG44 host cell lines capable of expressing novel bi-functional fusion proteins developed using Agonist Redirected Checkpoint (ARC)
  • Author regulatory documentations to support use of the CHO-DG44 host cells for CLD
  • Collaborate with other CLD scientist to establish a high yielding stable cell line development platform

 

Required Qualifications

  • Ph.D. in cell and molecular biology preferably with 1-2 years postdoctoral experience
  • Experience in CHO cell engineering and cell line development in biotechnology industry
  • Hands on experience in cutting-edge cell line development technologies to optimize platforms and shorten cycle time
  • Experience in CHO culture media evaluation for static, shaken conditions for both small and large scale expression
  • Proficient and hands-on experience with

Transfection (stable & transient)

Single cell cloning and monoclonality documentation

Analytical method development to support CLD including ELISA and MSD

Molecular methods including qRT-PCR, gene expression and gene editing

Flow cytometry

Robotic single cell cloning methods including ClonePix and FACS

  • Execute parental CHO-DG44 cell line adaptation and assess metabolic and genomic stability of the host cell lines
  • Adapt host cells to support early stage therapeutic protein discovery efforts using rapid transient gene expression
  • Write technical reports and standard operating procedure to support host cell line development and cell banking to meet ICH guidelines
  • Must enjoy working in collaboration with others and in small teams to achieve goals through building relationships with co-workers

 

 

 

 

 

 

 

 

 

Note to External Recruiters: Our Human Resources department manages all open positions and candidate recruitment. We kindly ask that recruiters not contact employees or hiring managers directly to present candidates or solicit business. Please note that we will consider failure to comply with this request when determining whether to enter into a professional relationship with a recruiting agency. Please also note that submission of unsolicited resumes does not create any obligations for Shattuck Labs, implied or otherwise.