Spirovant is a gene therapy leader focused on changing the course of cystic fibrosis and other respiratory diseases. Our gene therapy technologies are using adeno-associated virus (AAV) and lentivirus vectors designed to overcome the historical barriers that have prevented effective genetic treatments for inherited lung conditions such as cystic fibrosis. We are a rapidly growing company located in the biotech heart of University City, Philadelphia. To support our continued expansion and enable our future success, Spirovant is looking for a Research Associate, Upstream.

The Research Associate, Upstream will be responsible for performing a variety of laboratory procedures related to the production of gene therapy vectors using mammalian cells. This individual will join a dynamic, rapidly growing, and highly collaborative team focused on the development of gene therapies for respiratory diseases.

Key Responsibilities:

• Perform various bench scale procedures including buffer and media preparation, centrifugation, and filtration.
• Execute upstream operations for production of viral vectors (AAV and LVV) by performing techniques such as transfection and supernatant harvest.
• Set up and maintenance of various cell cultures using aseptic technique
• Perform various analytical procedures needed to support Vector Core activities. This includes but is not limited to SDS-PAGE, ELISA and Western blotting. Perform data analysis as needed.
• Participate on multi-disciplinary teams and communicate results and procedures (i.e., presentations, writing reports).
• Record all experiments and procedures in the Electronic Laboratory Notebook system in a timely and accurate fashion.

Preferred Qualifications:

• Familiarity with basic laboratory procedures including pipetting and aseptic technique.
• Basic knowledge and application of theories, principles and techniques in cell culture and vector production.
• Experience working with bioreactors up to 10L, CellFactories/CellStacks, viral vectors and performing various analytical techniques such as SDS-PAGE and western blotting is preferred.
• Familiarity with downstream procedures such as chromatography and molecular biology procedures such as qPCR is a plus.
• Experience working with Electronic Laboratory Notebooks (ELN) and inventory systems.

Basic Qualifications:

• BS, MS or equivalent in a science program preferred (e.g. biology, chemistry, etc.)
• 2-5 years of relevant laboratory experience, in academic, pharmaceutical or biotechnology settings.
• Attention to detail and problem-solving skills.
• Motivated, curious, and comfortable working with highly technical projects and personnel.
• Excellent communication and presentation skills, capable of conveying technical information in a clear and thorough manner.
• Comfortable delivering against challenging commitments.
• Confident, execution focused, team oriented, with an ability to thrive in an entrepreneurial environment.

Spirovant is committed to fostering a diverse, inclusive environment and is proud to be an equal opportunity employer.